Induction of direct somatic embryogenesis and callogenesis in date palm (Phoenix dactylifera L.) using leaf explants
نویسندگان
چکیده
منابع مشابه
Somatic embryogenesis from bud and leaf explants of date palm (Phoenix dactylifera L.) cv. Najda
An efficient regeneration system through somatic embryogenesis was developed for date palm cv. Najda. Adventitious bud and proximal leaf segments cultured on Murashige and Skoog (MS) medium supplemented with various combinations of auxins and cytokinins induced embryogenesis after at least 6 months of culture. Somatic embryogenesis induction seemed correlated with the type of the explant, the i...
متن کاملSomatic embryogenesis in date palm (Phoenix dactylifera L.) 'Deglet Nur' and 'Takerbucht' cultivars
The date palm has a pivotal role in the oasis ecosystem and it is an important food crop and commercial commodity. The cloning or protoplast fusion can increase breeding and multiplication of many varieties. Present investigation has been undertaken with the aim to develop methods for obtaining plantlets by somatic embryogenesis either from vegetative or female floral explants, as well as by th...
متن کاملInfluence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactylifera L.) Sahelian Cultivars
This study provides a physiological analysis of somatic embryogenesis in four elite cultivars of date palms: Ahmar, Amsekhsi, Tijib, and Amaside, from the initial callogenesis to establishment and proliferation of embryogenic suspension cultures. Somatic embryos development and in vitro plants rooting were also studied. For each step, auxins and cytokinins concentrations were optimised. The pri...
متن کاملA simplified Protocol to Induce Callogenesis in Protoplasts of Date Palm (Phoenix dactylifera L.) Cultivars
Background: In Algeria, date palm is currently confronted to the Bayoud disease. Biotechnological tools such as protoplasts fusion can appear as an alternative to ensure rapid multiplication and improvement of this species. Objectives: Callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars. Materials and Methods: Some factors influencing the isolatio...
متن کاملRapid in vitro regeneration of date palm (Phoenix dactylifera L.) cv. Kheneizi using tender leaf explant
Somatic embryogenesis has been accomplished from tender leaf base explant of date palm (Phoenix dactylifera L.). Three to five mm long tender leaf base explants derived from the meristamatic region of 2-3 year old offshoots of date palm cv. Kheneizi were cultured on Murashige and Skoog (MS) basal medium supplemented with 10, 50, 100 and 150 mg l 2,4-dichlorophenoxy acetic acid (2,4-D) and incub...
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ژورنال
عنوان ژورنال: BioTechnologia
سال: 2018
ISSN: 0860-7796
DOI: 10.5114/bta.2018.77480